Advantageously, the modification is only transiently present during the selection cycle. This enable a high degree of compatibility with the enzymatic step of the selection process. This approach further allows introducing larger chemical entities into DNA libraries that cannot be used using current methods.
The click chemistry has a broad acceptance of diverse substrates, which are either commercially available or accessible by chemical synthesis.
On behalf of University of Bonn, PROvendis offers licenses for commercial use as well a research collaboration with licensing option.
An International and a European patent application are pending:
International Patent Application WO 2015/049359
International Patent Application WO 2016/050850
Mayer, G., et al. (2016) Click reaction on solid phase enables high fidelity synthesis of nucleobase-modified DNA. Bioconjug. Chem. Feb 5., epub ahead of print.
Dellafiore, M.A., et al. (2016) Modified nucleoside triphosphates for in-vitro selection techniques. Front. Chem. 4: 19, epub.
Tolle, F., et al. (2015) A versatile approach towards nucleobase-modified aptamers. Angew. Chem. Int. Ed. Engl. 54(37): 10971-4.