Downstream processing of L-Tryptophan - Efficient preparation and purification of L-Tryptophan
Keywords: Aufreinigung von L-Tryptophan aus biotechnologischer Herstellung, Purification of L-Tryptophan from biotechnological processes
A prominent method for its preparation is the biotechnological fermentation with microorganisms. The prepared raw Tryptophan still includes impurities and has therefore be purified before it can be used in the described areas. Known purification methods use the crystallisation effect of Tryptophan under change in the pH. In order to use this method, the fermentation broth has first to be treated with sulfuric acid to reach a pH below 2 and then the pH has to be raised rapidly by using e.g. sodium hydroxide to end at a pH around 6-8. This process has several disadvantages. Besides the loss of acid and base in high volumes, the usage and combination of both come along with corrosive processes. Therefore, the construction and equipment has to be selected with respect to this. The corresponding salts have to be removed in washing steps from the crystals, too. The presented invention here avoids the usage of acids and bases and uses specific alcohols in a cooling process instead. The proof of principle has been shown in a batch and also continuous processes. The combination of process steps as solving of the product, reduction of solvent and cooling of the broth can be varied and show different effects of the characteristics and accessible yields of Tryptophan.
- Acid and base free purification of Tryptophan
- Easy handling of solvents
- Up to 90% recovery
- Reduction of waste material
- Varied crystals under specific conditions
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Hohmann, L. et al. (2016) Design of a Continuous Tubular Cooling Crystallizer for Process Development on Lab‐Scale. Chem. Eng. Technol. 39(7): 1268-80 doi.org/10.1002/ceat.201600072.
An invention of TU Dortmund.