Gfi1(1b/1b) and Gfi1(P2A/P2A) Mice
Ref.-No. 2960 - 3947
The Snail/Gfi1 (SNAG) family of zinc finger proteins is a group of transcriptional repressors. Gfi1 is a transcriptional repressor being expressed in the hematopoietic and nervous system. Loss of function leads to severe defects in hematopoiesis and inner ear development.
Gfi1(P2A/P2A): Deafness caused by defects of the hair cell formation in the inner ear, impaired erythroid and megakaryocytic hematopoiesis.
Gfi1(1b/1b): These mice do not exhibit significant defects in the hematopoietic system as the Gfi1(P2A/P2A)-mice do, suggesting that Gfi1b is in part able to rescue the loss of Gfi1-function.
Gfi1(P2A/P2A): A point mutation has been inserted in the SNAG domain of the Gfi1 gene that replaces a proline at amino acid position 2 by alanine (P2A). This completely abrogates the activity of Gfi1 as transcriptional repressor.
Gfi1(1b/1b): Gfi1 is highly similar to its paralogue Gfi1b with almost identical sequences in their respective protein domains. A knock-in mouse model was created in which the Gfi1 coding region was replaced by Gfi1b.
Research, drug development
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