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Long-term culture associated changes need to be considered for quality control of cell preparations. The invention discloses a simple method to track cellular aging based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic signature can be used as a biomarker for various cell types to predict
– continuously over the time – the state of cellular aging with regard to the number of passages or days of in vitro culture.
Cross-contaminated and misidentified cell lines are a major problem in cell
biology. In analogy, cross-contamination or mixing up of donor samples are also problematic for primary cells. Quality control is even more important for cellular therapy – the use of early passages is usually recommended due to less functional changes and less accumulation of mutations.
Keywords: Zellkultur, Langzeitkultur, Qualitätskontrolle, Zellen, zelluläres Altern, Seneszenz, Epigenetik, DNA-Methylierung, Cell culture, long-term culture, quality control, cells, cellular aging, senescence, epigenetics, DNA-methylation
Scientists of the RWTH Aachen developed a simple epigenetic test to track cellular aging. The method does not require a reference-sample of the corresponding early passage and provides a simple quality control to determine the state of cellular aging.
As induced pluripotent stem cells (iPS) do not reveal cellular aging and are predicted passage zero, the identification of high quality induced iPS might also be possible.
On behalf of RWTH Aachen, PROvendis offers access to rights for commercial use as well as the opportunity for further co-development.
In case of interest we are pleased to inform you about the current patent status.