The inventors of the present invention identifed the cAMP nucleotide-binding domain of the bacterial MlotiK1 channel as superior cAMP sensing moiety for a FRET-based cAMP sensor. Sandwiched between a blue and a yellow fluorescent protein, this motif exhibits a nanomolar affinity and allows for real-time mesurement of intracellular cAMP concentration. The inventors were able to monitor the cAMP dynamics in different cell types, e.g. HEK293 cells, but also in flagella of sperm, thereby revealing the spatio-temporal distribution of cAMP controlling flagellar movement. This work, please find the reference below, proofs the applicability of the presented cAMP sensor for the investigation of cAMP-related biological questions.
The invention is available for licensing.
- Nanomolar affinity
- Fast binding/unbinding kinetics
- Expressed and purified in high amounts from prokaryotic cells
- Well expressed in eukaryotic cells.
- Measurement of cAMP levels in solution in vitro, in cellulo, and in vivo
- No interaction with molecular pathways of eukaryotic cells
- Amenable to subcellular targeting
The invention is described and claimed in the PCT application WO 2017/045970 A1 filed 09/06/2016.
The national german counterpart DE 10 2015 115 640 B4 filed 09/16/2015 has been granted. This application is the priority application to said PCT application. The patent protects the use of a procaryotic cAMP nucleotide-binding domain for use in a biosensor.
Mukherjee et al.: eLife 2016;5:e14052 DOI: 10.7554/eLife.14052