This novel broad host range expression vector can be equally well used in gram(+) and gram(-) hosts. Thus, this new tool may be used to establish environmental DNA (eDNA) expression libraries and to screen for desired activities in different gram(+) as well as gram(-) hosts at the same time. The vector may allow finding novel biocatalysts, which are not functional in the limited number of vector compatible hosts so far.
The present invention provides a novel broad host range expression vector for establishing expression libraries with unknown DNA sequences like eDNA libraries.
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