Antibody against Amyloid-beta phosphorylated at Ser 8 and 26
The antibodies are directed against specific phosphorylation sites of Amyloid-beta at serine 8 or at serine 26, respectively. Forms of Amyloid beta phosphorylated at these serine residue have increased propensity to form toxic aggregates as compared to non-phosphorylated species.
A: The aminoterminal peptide 1-14 of human Amyloid-beta: DAEFRHD(phosphorylated-S)GYEVHHC
B: the non-phosphorylated peptide used in A
C: Amino acids 20-33 of the human Amyloid-beta: FAEDVG(phosphorylated-S)NKGAIIG
The peptides were conjugated with keyhole limpet hemocyanin (KLH) as an immunogen. The antibodies bind to unaggregated and aggregated human Amyloid beta.
A: Mouse monoclonal
B: Rat monoclonal
C: Mosue monoclonal
ELISA, Western Blot, Immunohistochemistry
A and B: Kumar, S., et al. (2013) Early intraneuronal accumulation and increased aggregation of phosphorylated Abeta in a mouse model of Alzheimer’s disease. Acta Neuropath. 125: 699-709.