The antibodies are directed against specific phosphorylation sites of Amyloid-beta at serine 8 or at serine 26, respectively. Forms of Amyloid beta phosphorylated at these serine residue have increased propensity to form toxic aggregates as compared to non-phosphorylated species.

Immunogen/Epitope
A: The aminoterminal peptide 1-14 of human Amyloid-beta: DAEFRHD(phosphorylated-S)GYEVHHC

B: the non-phosphorylated peptide used in A

C: Amino acids 20-33 of the human Amyloid-beta: FAEDVG(phosphorylated-S)NKGAIIG

The peptides were conjugated with keyhole limpet hemocyanin (KLH) as an immunogen. The antibodies bind to unaggregated and aggregated human Amyloid beta.

Antibody-Type
A: Mouse monoclonal

B: Rat monoclonal

C: Mosue monoclonal

Usage
Research

Tested Applications
ELISA, Western Blot, Immunohistochemistry

Reference
A and B: Kumar, S., et al. (2013) Early intraneuronal accumulation and increased aggregation of phosphorylated Abeta in a mouse model of Alzheimer’s disease. Acta Neuropath. 125: 699-709.