This invention allows an easy and economical way for the stratification of patients (e.g. for treatment with tyrosine-kinase-inhibitors) with unique sensitivity. Based on RT-PCR, the technical requirements are conventional and worldwide accessible. The non-invasive analysis of free DNA from plasma/serum samples is possible and facilitates continuous monitoring of treatment response.
Platform technology established for common oncogenic mutations with similar impressive sensitivities - BRAF (V600E), EGFR (DelEx19, L858R, T790), KRAS (codon 12/13/61).
On behalf of University of Duisburg-Essen, PROvendis offers access for commercial use in terms of a license as well as research collaborations. In case of interest, we are pleased to inform you about the current patent status.
A European and a US patent have already been granted, other applications are pending.
Breitenbuecher et al. (2014) Development of a highly sensitive and specific method for detection of circulating tumor cells harboring somatic mutations in non-small-cell lung cancer patients. PLoS One 9(1):e85350
Oezkan et al. (in preparation 2016): Rapid, cost-effective and highly sensitive mutation detection in EBUS-TBNA specimen of lymph node metastases in patients with lung adenocarcinoma.