Life Sciences

Life Sciences

VisuVivo - A proliferation marker to visualise cell cycle progression in vitro and in vivo with high spatial resolution of the M-phase



Ref.-Nr. 2092

The invention provides a nucleic acid expression construct encoding a fusion protein comprising a fluorescence reporter protein (like EGFP) and a protein with a wild-type destruction signal (like Anillin). Localised to subcellular structures during cell cycle progression, it presents a fluorescence marker for imaging cell cycle progression in vitro and in vivo.

The cell cycle comprises consecutive phases termed G1, S (synthesis), G2 (interphase) and M (mitosis). Cells that temporarily or reversibly stop dividing enter quiescence, named the G0-phase. To differentiate between cells that start to divide again and resting cells is still an unreached goal. In addition, available cell cycle indicators are unable to distinguish between cell division and acytokinetic mitosis which is karyokinesis without cyotkinesis or endoreplication which is continuing rounds of DNA replication without karyokinesis.

Keywords: Proliferationsmarker, Visualisierung, in vivo, Zellzyklus, Mitose, Proliferation, Progression, Fluoreszenz, Luminsezenz, EGFP, Regenerative Medizin, in vitro, M-Phase, Proliferation, marker, visualization, cell cycle, mitosis, fluorescence, luminescence, regenerative medicine, in vitro, acytokinetic, karyokinesis, endoreplication

The Anillin fusion protein of the invention is located in the nucleus during G1-, S- and G2-phase, in the cytoplasm and cell cortex in early M-Phase, in the contractile ring during cytokinesis and in the midbody just before absission, making it possible to distinguish between different phases. At the end of mitosis the fusion protein gets degraded by the proteasome. Therefore, cells arrested in G0 do not show any fluorescence.

The invention enables scientists to identify, isolate and characterize proliferating cells out of a composition of proliferating, differentiated and post-mitotic cells. Furthermore, it enables to distinguish between cell division and variations of the cell cycle such as acytokinetic mitosis and endoreplication that cause false positives in standard proliferation assays.

In case of interest we are pleased to inform you about the current patent status. We offer access to rights for commercial use as well as the opportunity for further co-development.

Stable transfected, pluripotent murine embryonic stem cells were generated. VisuVivo can aid to gain knowledge about mechanisms of cell proliferation, which could be useful for stem cell biology as well as regenerative medicine and others.

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